What is the application of the rotary evaporator?

What is the application of the rotary evaporator?

What is the application of the rotary evaporator?

Rotary evaporation instruments are mainly used for concentration, crystallization, drying, separation and solvent recovery in the pharmaceutical, chemical and biopharmaceutical industries. The principle is as follows: under vacuum condition, the constant temperature is heated, so that the rotating bottle rotates at a constant speed, and the material forms a large-area film on the bottle wall and evaporates. The solvent vapor is cooled by the glass condenser and recovered in the collection bottle, thereby greatly improving the evaporation efficiency, and is especially selected for the concentration and purification of the biological product which is easily decomposed and degenerated by high temperature.

Rotary evaporators have the following main features:

1. All rotary evaporators have a built-in lift motor that automatically raises the flask to a position above the heating pot when the power is off.
2. Due to the centripetal force and friction between the liquid sample and the evaporation bottle, the liquid sample forms a liquid film on the inner surface of the evaporation bottle, and the heat receiving area is large;
3. The force generated by the rotation of the sample effectively suppresses the boiling of the sample. The above features and its convenient features make the modern rotary evaporator available for rapid and gentle distillation of most samples, even for operators with no operational experience.

The biggest drawbacks of rotary evaporator applications:
It is the boiling of certain samples, such as ethanol and water, which will cause the experimenter to collect the loss of the sample. In operation, it is usually possible to prevent boiling by carefully adjusting the working strength of the vacuum pump or the temperature of the heating pot during the mixing phase of the distillation process. Alternatively, it is also possible to add anti-boiling particles to the sample. For samples that are particularly difficult to distill, including samples that are prone to foaming, a special condenser can also be configured for the rotary evaporator.

Principle: An evaporation instrument widely used in laboratories. Suitable for reflow operations, rapid evaporation of large amounts of solvent, concentration of minor components, and reaction processes requiring agitation. The rotary evaporator system can seal the pressure to 400-600 mmHg; the solvent in the distillation flask is heated by a heating bath, the heating temperature can be close to the boiling point of the solvent; and the rotation can be performed at a speed of 50-160 rpm. The solvent is formed into a film to increase the evaporation area. In addition, under the action of a high-efficiency cooler, the hot vapor can be quickly liquefied to accelerate the evaporation rate.

Uses: Rotary evaporators are mainly used for concentration, crystallization, drying, separation and solvent recovery in the pharmaceutical, chemical and biopharmaceutical industries. The principle is that under vacuum condition, the temperature is heated at a constant temperature, so that the rotating bottle rotates at a constant speed, and the material forms a large-area film on the bottle wall, and evaporates efficiently. The solvent vapor is cooled by a high-efficiency glass condenser and recovered in a collection bottle to greatly increase the evaporation efficiency. It is especially suitable for the concentration and purification of biological products which are easily decomposed and degenerated by high temperature.

Definition: A device for concentrating a fast liquid sample. The sample is heated, depressurized, and continuously rotated in a spherical glass vessel to increase the evaporation surface area and accelerate the evaporation rate.
As the production technology of laboratory equipment in the world has become more mature, more and more university laboratories have begun to use rotary evaporators. The rotary evaporator is a device that uses a rotary evaporation bottle to increase the evaporation area while being placed in a water bath under a reduced pressure while heating, and the solution in the bottle is diffused and evaporated. Next, NewLabX will talk about the role of rotary evaporator, the factors affecting distillation efficiency and the scope of application.

1. Rotary evaporator function
Rotary evaporators are primarily used to continuously distill large amounts of volatile solvents under reduced pressure. In particular, the concentration of the extract and the distillation of the receiving liquid in the chromatographic separation can separate and purify the reaction product. The basic principle of a rotary evaporator is vacuum distillation, that is, under reduced pressure, the distillation flask is continuously rotated as the solvent is distilled.
Factors affecting distillation efficiency of rotary evaporator
2. Vacuum degree of the rotary steaming system
1 Rotary evaporation evaporator is composed of vacuum pump and its pipeline, evaporation bottle and receiving bottle, condenser tube and other components. Among them, the key factors affecting the degree of vacuum are: vacuum pump power, system tightness. Among them, the equipment includes: a vacuum pump, a sealing ring and a vacuum tube.
2 The lower the vacuum pump limit, the lower the vacuum value of the system. However, the vacuum value that the system can form is determined by the pressure resistance of the equipment of the rotary evaporator and the avoidance of bumping when the distillation efficiency is ensured. To set a reasonable vacuum value, it is necessary to combine the above two factors and adjust it by the vacuum controller.
3 sealing ring: connecting the key parts between the evaporation tube and the condensation tube, wear resistance and corrosion resistance are the most important performance requirements. Common materials are PTFE and rubber. Among them, PTFE has better wear resistance and corrosion resistance than rubber.
4 Vacuum pumps and seals are all wearable parts. The vacuum pump should be tested frequently during use to ensure the stability of its vacuum. The seal ring is a detachable accessory that can be replaced directly. Vacuum tubes often use silicone tubing instead of rubber because of their slow aging.

Rotary heating power
The higher the temperature of the oil bath, the faster the distillation rate of the solvent. In the actual distillation process, distillation efficiency is often not pursued by setting an excessively high reaction temperature. Reasons include:
1 If the distilled substance is a heat sensitive substance, the high temperature is easily decomposed and deteriorated;
2 Excessive temperature can easily lead to high temperature softening of seals and other components, reducing their service life and reducing the tightness of the system. Typically, the oil bath is heated in a water bath with a temperature setting below 60 °C. The temperature is higher than 80 ° C and heated in an oil bath.
3 motor speed

The faster the motor speed, the larger the area of ​​the evaporation bottle, the larger the heat receiving area, the thicker the liquid film formed, the higher the heat transfer temperature difference, and the higher the distillation efficiency. In actual use, the speed is not as fast as possible. It is mainly due to:
1 The faster the rotation speed, the greater the centrifugal force formed, which easily leads to the drop of the bottle;
2 The viscosity of the material is different, there is an investigation of the rotational speed.
4 cooling system

The distillation efficiency is better, the temperature of the cooling system is kept at a temperature difference of 40 ° C from the temperature of the heating pot, so that the formed hot steam is rapidly condensed to reduce the influence of steam on the vacuum degree of the system. A commonly used cooling medium is circulating condensate. If there is a substance with a particularly low boiling point, an ice water bath and an ice ethanol bath using a circulating refrigerator as a carrier are also common choices.

Rotary evaporator application range
The rotary evaporator is a device that uses a rotary evaporation bottle to increase the evaporation area while being placed in a water bath under a reduced pressure while heating, and the solution in the bottle is diffused and evaporated. It is an ideal basic instrument for chemical industry, pharmaceutical industry, universities and research laboratories for the production and analysis of experiments, such as concentration, drying and recycling.

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Créer un ici", "forgot": "Mot de passe oublié", "all_collection": "Toutes les collections", "world_wide_delivery": "Livraison à l'échelle mondiale", "shipping_text": "Livraison gratuite au Royaume-Uni sur des commandes de plus de 100 £", "hot_line": "Hot line" }, "menu": { "mobile_menu_tab": "Menu", "mobile_account_tab": "Compte", "mobile_settings_tab": "Paramètres" }, "slideshow": { "next_slide": "Diapositive suivante", "previous_slide": "Diapositive précédente", "pause_slideshow": "Mettre en pause le diaporama", "play_slideshow": "Jouer le diaporama", "play_video": "Lire la vidéo", "close_video": "Fermer la vidéo" }, "map": { "get_directions": "Obtenir des directions", "address_error": "Vous ne trouvez pas cette adresse", "address_no_results": "Aucun résultat pour cette adresse" } }, "blogs": { "article": { "view_all": "Voir toutes", "all_topics": "Tous les sujets", "by_author": "par [[ author ]]", "posted_in": "Publié dans", "read_more": "Plus", "back_to_blog": "Retour à [[ title ]]" }, "comments": { "title": "Laissez un commentaire", "name": "Nom", "email": "Courriel", "message": "Message", "post": "Publier le commentaire", "moderated": "Veuillez noter que les commentaires doivent être approvés avant d'être affichés", "success_moderated": "Votre commentaire a été soumis avec succès. Nous le publierons sous peu, suite à notre processus de modération.", "success": "Votre commentaire a été publié avec succès!", "comments_with_count": { "one": "[[ count ]] commentaire", "other": "[[ count ]] commentaires" } } }, "cart": { "general": { "title": "Panier", "note": "Ajouter une note à votre commande", "remove": "Retirer", "subtotal": "Sous-total", "savings": "Vous économisez", "shipping_at_checkout": "Frais de port et remises calculés à la caisse", "update": "Mettre à jour", "checkout": "Procéder au paiement", "empty": "Votre panier est vide.", "cookies_required": "Activer les cookies pour utiliser le panier", "cancel": "Annuler", "edit": "Éditer", "continue_shopping": "Continuer vos achats", "view_and_edit_cart": "Afficher et modifier le panier", "processing": "En traitement...", "items_count_label" : "[[ count ]] article(s) dans votre panier", "ok" : "D'accord", "empty_page_title": "Le panier d'achat est vide", "empty_continue_html": "Cliquer ici pour poursuivre vos achats.", "clear": "Vider le panier", "recently_added_item": "Article (s) récemment ajouté (s)", "remove_item": "Enlever cet article", "here": "ici" }, "label": { "product": "Produit", "price": "Prix", "quantity": "Quantité", "total": "Total", "total_item": "Total de l'élément", "sub_total_top": "Sous-total du panier" } }, "collections": { "general": { "view_all": "Voir toutes", "clear_all": "Tout Supprimer", "no_matches": "Aucun produit ne correspond à votre recherche.", "items_with_count": { "one": "[[ count ]] item", "other": "[[ count ]] items" }, "load_more": "Charger plus", "sidebar_btn": "Filtrer par" }, "sorting": { "title": "Trier par", "manual": "En vedette", "best_selling": "Meilleurs vendeurs", "title_ascending": "A-Z", "title_descending": "Z-A", "price_ascending": "Prix: faible à élevé", "price_descending": "Prix: élevé à faible", "created_descending": "Date: récent à ancien", "created_ascending": "Date: ancien à récent" }, "filters": { "title_tags": "Filtrer", "all_tags": "Tous les produits", "categories": "Les catégories", "title": "Filtre", "color": "Couleur", "size": "Taille", "brand": "Marque", "price": "Prix", "green": "Vert", "blue": "Bleu", "red": "Rouge", "pink": "Rose", "black": "Noir", "purple": "Violet", "white": "Blanc", "orange": "Orange" }, "product_item": { "quick_shop": "Aperçu rapide", "compare": "Comparer", "wishlist": "Ajouter à la liste de souhaits" } }, "contact": { "form": { "name": "Nom", "email": "Courriel", "phone": "Téléphone", "message": "Message", "submit": "Envoyer", "post_success": "Merci de nous avoir avoir contacté. Nous vous reviendrons le plus rapidement possible.", "address": "Adresse", "telephone": "Téléphone", "title": "Écrivez-nous", "required": "Requis" } }, "customer": { "account": { "title": "Mon compte", "details": "Détails du compte", "view_addresses": "Voir les adresses", "return": "Retour aux détails du compte" }, "activate_account": { "title": "Activer le compte", "subtext": "Créez votre mot de passe pour activer le compte.", "submit": "Activer le compte", "cancel": "Refuser l'invitation", "password": "Mot de passe", "password_confirm": "Confirmer le mot de passe" }, "addresses": { "title": "Votre adresse", "default": "Par défaut", "add_new": "Ajouter une nouvelle adresse", "edit_address": "Éditer l'adresse", "first_name": "Prénom", "last_name": "Nom", "company": "Compagnie", "address1": "Adresse 1", "address2": "Adresse 2", "city": "Ville", "country": "Pays", "province": "Province", "zip": "Code postal", "phone": "Téléphone", "set_default": "Définir comme adresse par défaut", "add": "Ajouter l'adresse", "update": "Mettre à jour l'adresse", "cancel": "Annuler", "edit": "Éditer", "delete": "Supprimer", "delete_confirm": "Êtes-vous certain(e) de vouloir supprimer cette adresse?" }, "login": { "title": "Connexion", "desc": "Si vous avez un compte, connectez-vous avec votre adresse e-mail.", "email": "Courriel", "password": "Mot de passe", "forgot_password": "Mot de passe oublié?", "sign_in": "Se connecter", "guest_title": "Continuer en tant qu'invité", "guest_continue": "Continuer" }, "orders": { "title": "Historique des commandes", "order_number": "Commande", "date": "Date", "payment_status": "Statut du paiement", "fulfillment_status": "Statut du traitement de la commande", "total": "Total", "none": "Vous n'avez pas placé de commande à ce jour." }, "order": { "title": "Commande [[ name ]]", "date": "Placée le [[ date ]]", "cancelled": "Commande annulée le [[ date ]]", "cancelled_reason": "Motif: [[ reason ]]", "billing_address": "Adresse de facturation", "payment_status": "Statut du paiement", "shipping_address": "Adresse de livraison", "fulfillment_status": "Statut du traitement de la commande", "discount": "Rabais appliqué", "shipping": "Livraison", "tax": "Taxes", "product": "Produit", "sku": "SKU", "price": "Prix", "quantity": "Quantité", "total": "Total", "fulfilled_at": "Traitée le [[ date ]]", "subtotal": "Sous-total" }, "recover_password": { "title": "Réinitialiser votre mot de passe", "email": "Courriel", "submit": "Soumettre", "cancel": "Annuler", "subtext": "Nous vous ferons parvenir un courriel pour réinitialiser votre mot de passe.", "success": "Nous vous avons fait parvenir un courriel pour réinitialiser votre mot de passe." }, "reset_password": { "title": "Réinitialiser le mot de passe du compte", "subtext": "Entrez un nouveau mot de passe pour [[ email ]]", "submit": "Réinitialiser le mot de passe", "password": "Mot de passe", "password_confirm": "Confirmer le mot de passe" }, "register": { "title": "Créer un compte", "first_name": "Prénom", "last_name": "Nom", "email": "Courriel", "submit": "Créer", "password": "Mot de passe", "desc": "La création d'un compte est simple. Remplissez le formulaire ci-dessous." } }, "homepage": { "onboarding": { "product_title": "Le nom de votre produit", "product_description": "Cette partie est utilisée pour la fiche du produit. Parlez aux clients de l'allure, du ressenti et du style de votre produit. Ajoutez des détails sur la couleur, les matériaux utilisés, le dimensionnement, et où il a été fabriqué.", "collection_title": "Le nom de votre collection", "blog_title": "Le titre de votre publication", "blog_excerpt": "Votre magasin n'a encore rien bloggué. Un blog peut être utilisé pour parler des lancements de nouveaux produits, d'astuces, ou d'autres nouvelles que vous voulez partager avec vos clients. Vous pouvez regarder le blog d'e-commerce de Shopify pour trouver de l'inspiration et des conseils pour votre propre magasin et blog.", "blog_author": "Nom de l'auteur", "no_content": "Cette section ne contient actuellement aucun contenu. Ajoutez-en en utilisant la barre latérale." } }, "layout": { "navigation": { "search": "Recherche", "toggle": "développer\/réduire", "expand": "révéler", "collapse": "fermer", "all_categories": "toutes catégories" }, "cart": { "title": "Panier", "items_count": { "one": "item", "other": "items" } }, "customer": { "account": "Compte", "log_out": "Se déconnecter", "logout": "Se déconnecter", "log_in": "Se connecter", "create_account": "Créer un compte", "sign_up": "S'inscrire", "wishlist": "Liste de souhaits" }, "footer": { "social_platform": "[[ name ]] sur [[ platform ]]" }, "list_page": { "grid": "La grille", "list": "Liste" } }, "products": { "product": { "regular_price": "Prix régulier", "sold_out": "Épuisé", "unavailable": "Non disponible", "on_sale": "Solde", "quantity": "Quantité", "add_to_cart": "Ajouter au panier", "back_to_collection": "Retour à [[ title ]]", "related_title": "Produits connexes", "qty_increase": "Augmenter", "qty_decrease": "Diminution", "deal_days": "Journées", "deal_hours": "Heures", "deal_minutes": "Minutes", "deal_second": "Seconde", "select_option": "Sélectionner une option", "add_to_wishlist": "Ajouter à la liste de souhaits", "add_to_review": "Ajouter à la critique", "first_review": "Soyez le premier avis", "compare_success_msg": "[[ product_title ]] a ajouté à la boîte de comparaison réussie", "compare_exist_msg": "[[ product_title ]] existe dans la boîte de comparaison", "compare_cart_msg": "[[ product_title ]] a ajouté à la liste de souhaits réussie", "compare_remove_msg": "[[ product_title ]] a été supprimé de la boîte de comparaison", "comparing_box": "Boîte de comparaison", "compare_no_items": "Il n'y a aucun élément dans la boîte de comparaison", "wishlist_success_msg": "[[ product_title ]] a ajouté à la liste de souhaits réussie", "wishlist_exist_msg": "[[ product_title ]] existe dans la liste de souhaits", "wishlist_cart_msg": "[[ product_title ]] a ajouté au panier", "wishlist_box": "Liste de souhaits", "wishlist_remove_msg": "[[ product_title ]] a été retiré de la liste de souhaits", "wislist_no_items": "Il n'y a aucun élément dans la liste de souhaits", "upsell_cart_msg": "\"[[ product_title ]]\" a ajouté à la liste de souhaits réussie", "upsell_block_title": "Souvent acheté avec \"[[ product_title ]]\"", "upsell_cart_qty": "[[ count ]] article", "upsell_product_page_title": "Vous aimerez peut-être aussi ces produits", "upsell_checkout_btn": "Caisse", "share": "Partager le produit", "share_on_facebook": "Partager sur Facebook", "share_on_twitter": "Partager sur Twitter", "share_on_pinterest": "Partager sur Pinterest", "share_on_google": "Partager sur Google+", "share_on_linkedin": "Partager sur LinkedIn", "availability": "Disponibilité", "in_stock": "En stock", "out_of_stock": "En rupture de stock", "quick_overview": "Rapide vue d'ensemble", "details": "Détails", "reviews": "Avis", "tags": "Tags du produit", "options": "Les options", "vendor": "Vendeur", "features": "Les traits", "size_chart": "Tableau des tailles", "sale_left_text":"[[ sales ]] VENDU. SE DÉPÊCHER! SEULEMENT QUELQUES GAUCHE!", "checkout_text": "Paiement sécurisé et approuvé avec", "quick_view_details": "Voir les détails", "open_light_gallery": "Cliquez ici pour ouvrir les images de la galerie" }, "upsell": { "recommend_text": "Quelqu'un a acheté un", "minute_ago": "il y a quelques minutes" } }, "gift_cards": { "issued": { "title_html": "Votre carte-cadeau [[ shop ]] d'une valeur de [[ value ]]!", "subtext": "Voici votre carte-cadeau!", "disabled": "Désactivée", "expired": "Expirée le [[ expiry ]]", "active": "Expire le [[ expiry ]]", "redeem_html": "Entrez ce code lors du paiement pour utiliser votre [[ value ]] carte-cadeau", "shop_link": "Boutique", "print": "Imprimer ce bon d'achat", "remaining_html": "[[ balance ]] restant", "add_to_apple_wallet": "Ajouter à Apple Wallet" } }, "date_formats": { "month_day_year": "%d %B, %Y" } } }